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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Methods of murine brain 3D imaging focusing on tissue clearing
 Novák, Jaromír ; Drbal, Karel (advisor) ; Němec, Pavel (referee)
1 ABSTRACT Many variants of microscopic techniques are the key methods for tissue structure examination in the research as well as in clinical practice. Since the vast majority of examined tissues are intrinsically opaque, it is difficult to reconstruct their 3D structure with light microscopy. However the spatial structure is inherently connected to the function of cells, tissues or whole organs. The most common approach is sample sectioning to the thin layers, which are subsequently examined or used for 3D structure reconstruction of the sample. A different approach is to use a series of chemical reagents able to clear the samples and make them accessible for imaging via microscopy without the need for physical sectioning. Although this approach was first used more than a century ago, the widespread use of these methods came in the last few years. Since the resurgence of tissue clearing method a lot of new principles how to clear the samples were discovered as well as many new chemical compounds can be used for this purpose.. Clearing methods are becoming more and more sophisticated: the volume of samples which can be cleared is continuously growing, new methods are becoming faster and automated, the quality of after-cleared tissues is improving and compatibility with many stains including antibodies is...
Detailed record
Methods of murine brain 3D imaging focusing on tissue clearing
 Novák, Jaromír ; Drbal, Karel (advisor) ; Němec, Pavel (referee)
1 ABSTRACT Many variants of microscopic techniques are the key methods for tissue structure examination in the research as well as in clinical practice. Since the vast majority of examined tissues are intrinsically opaque, it is difficult to reconstruct their 3D structure with light microscopy. However the spatial structure is inherently connected to the function of cells, tissues or whole organs. The most common approach is sample sectioning to the thin layers, which are subsequently examined or used for 3D structure reconstruction of the sample. A different approach is to use a series of chemical reagents able to clear the samples and make them accessible for imaging via microscopy without the need for physical sectioning. Although this approach was first used more than a century ago, the widespread use of these methods came in the last few years. Since the resurgence of tissue clearing method a lot of new principles how to clear the samples were discovered as well as many new chemical compounds can be used for this purpose.. Clearing methods are becoming more and more sophisticated: the volume of samples which can be cleared is continuously growing, new methods are becoming faster and automated, the quality of after-cleared tissues is improving and compatibility with many stains including antibodies is...
Detailed record

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